ACCUMULATION OF GLYCOSAMINOGLYCANS AND OXYPROLINE IN THE KNEE JOINT OF RATS WITH DIFFERENT METHODS OF ADMINISTRATION OF PREPARATIONS THAT CONTAIN HUMIC SUBSTANCES

Abstract

Purpose. Identification of the most effective method of administering preparations based on humic substances in order to activate the accumulation of glycosaminoglycans and oxyproline within the structures of the knee joint in laboratory rats. Materials, methods. An experimental study was conducted on 30 sexually mature male laboratory rats. Animals were divided into 6 groups: group 1 – intact control; group 2 – drinking water and “Gumilid AS-10” 5 mg/kg + daily rubbing of 0.3 g “Gumipharm” ointment; group 3 – drinking water and “Gumilid AS-10” 5 mg/kg + electrophoresis with 0.3 g of “Gumipharm” ointment; group 4 – drinking water and “Gumilid AS-10” 5 mg/kg; group 5 – daily rubbing in of 0.3 g “Gumipharm” ointment; group 6 – electrophoresis and 0.3 g of “Gumipharm” ointment. Rubbing or electrophoresis with ointment was performed on the left knee joint. On day 21, the rats were taken out of the experiment under thiopental anesthesia, the left knee joints were isolated, homogenates were prepared, in which glycosaminoglycans and oxyproline were determined. Scientific novelty. In the experiment on laboratory animals, the effectiveness of the combined method of administering preparations containing humic substances without violating the integrity of tissues in order to activate the accumulation of glycosaminoglycans and oxyproline within the structures of the knee joint has been proven. Results. The conducted experimental study established the maximum accumulation of glycosaminoglycans in the knee joints of laboratory rats after oral administration of “Gumilid AS-10” in combination with local application of “Gumipharm” ointment by rubbing or electrophoresis. The level of glycosaminoglycans after using these methods of drug administration increased by 54.1 and 46.7 %, respectively. Oral administration of only “Gumilid AS-10” to animals increased this indicator in the knee joint by 16.0 %. The use of only local administration of drugs contributed to an increase in glycosaminoglycans in the knee joint of rats by 24.8 % (rubbing) and 29.3 % (electrophoresis). The accumulation of oxyproline in the knee joints of rats after different methods of introducing humic substances exhibited the same character. The level of oxyproline in the joint tissue increased by 60.6 % after oral administration + rubbing in the ointment, by 47.5 after oral administration + electrophoresis with ointment, by 12.1 % after oral administration, by 40.7 % after rubbing in the ointment and by 40.6 % after electrophoresis with ointment. Conclusion. The most appropriate way to activate the accumulation of glycosaminoglycans and oxyproline in the structures of the knee joint in laboratory rats under the influence of humic substances is the oral administration of “Gumilid AS-10” in combination with the local use of “Gumipharm” ointment by rubbing or electrophoresis.