CHANGES IN CONNECTIVE TISSUE METABOLISM IN BLOOD SERUM IN ALCOHOL PRODUCTION WORKERS WITH INFLAMMATORY-DYSTROPHIC DISEASES OF PERIODONTAL TISSUES
DOI:
https://doi.org/10.35220/2078-8916-2024-51-1.9Keywords:
generalized periodontitis, alcohol production, connective tissue metabolism, blood serum.Abstract
The aim of the study – to analyze changes in connective tissue metabolism in the blood serum of alcohol production workers with inflammatory and dystrophic diseases of periodontal tissues to determine further treatment tactics. Research methods. The study was performed at the Department of Therapeutic Dentistry, Periodontology and Dentistry, Danylo Halytsky Lviv National Medical University. The observation groups in our study were formed as follows: 50 people with generalized periodontitis (GP) who were directly employed in alcohol production – the main group; 20 people with GP who were not exposed to the negative factors of this production – a comparison group; 15 practically healthy people with clinically unchanged periodontal tissues – a control group. The study of collagenase activity in the blood serum of patients was performed by the Lindy method. Hydroxyproline fractions in the blood serum of the subjects were isolated by the method of Frey S., and the hydroxyproline content in them was determined by Stegeman H. F. The determination of glycosaminoglycans in the blood serum of patients was performed by the orcin method according to Klyatskin S. A. and Lifshitz R. I. The activity of alkaline phosphatase (ALP) in the blood serum of the study groups was determined using the Spektrum Series 2 autoanalyzer from Abbott (USA). Scientific novelty. Collagenolytic activity was increased in alcohol production workers with GP: the content of collagenase was significantly higher than in the control group (p<0,05) and in patients with GP of the comparison group (p<0,05), the concentration of protein-bound hydroxyproline decreased by 30,0 %, and in patients of the comparison group – by 14,67 %, compared to the control group (p<0,05). The analysis of the free fraction of oxyproline revealed a significant increase in the content of this fraction in the blood serum of patients with GP: in the main group by 20,0 % and by 6,0 % in the comparison group compared to the control group (p<0,05). In patients with GP of the main group, the content of glycosaminoglycans in the blood serum exceeded the control group by 105,88 % (p<0,05), and in the comparison group by 47,06 % (p<0,05). The dynamics of the content of alkaline phosphatase was characterized by an increase in the index in the main group by 136,47 % and by 55,29 % in patients of the comparison group (p<0,05). Conclusions. Thus, the analysis of connective tissue metabolism in patients with inflammatory-dystrophic diseases of periodontal tissues employed in alcohol production convincingly demonstrates a violation of the biosynthesis of the connective component of bone tissue and, accordingly, in the processes of metabolism of periodontal tissue construction and impaired reparative properties, which requires mandatory further pharmacotherapy with drugs with a directed osteotropic effect.
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