STUDY OF THE ANTI-INFLAMMATORY EFFICIENCY OF HYALURONIC ACID PREPARATIONS OF VARIOUS CONCENTRATION IN RATS WITH EXPERIMENTAL PARODONTITIS

Abstract

Among dental diseases, a significant proportion is occupied by periodontal diseases, which cause significant functional changes in the dental system, leading to a decrease in quality of life. In this regard, the search for new methods of prevention and treatment of periodontal diseases is important.
The aim this work was to study the anti-inflammatory efficacy of hyaluronic acid preparations of various concentrations (1.4 %; 2 %, 3 % and 4 %) with mannitol in rats with experimental parodontitis.
Materials and methods. The study was conducted on 96 Wistar rats of herd breeding, females age 6-7 months with an average weight of 285 ± 34 g. The animals were divided into 6 groups:
1. Intact
2. Parodontitis,
3. Parodontitis + 1.4 % non cross-linked hyaluronic acid + mannitol 0.9%,
4. Parodontitis + 2 % non cross-linked hyaluronic acid hyalu-ronic acid + mannitol 0.9 %,
5. Parodontitis + 3 % non cross-linked hyaluronic acid hyalu-ronic acid + mannitol 0.9 %,
6. Parodontitis + 4% non cross-linked hyaluronic acid hyalu-ronic acid + mannitol 0.9%.
Experimental pathology of parodontitis was reproduced by add-ing peroxidized sunflower oil to feed at the rate of 1 ml per ani-mal per day [1]. In the work non cross-linked hyaluronic acid hyaluronic acid with mannitol 0.9% drugs are used:
1.4 % – StylAge Hydro;
2 % – Hydryal, Luminera;
3 % – Hydryal, Luminera;
4 % – Hydryal, Luminera.
Injection of hyaluronic acid preparations was carried out once a day on the 21st day of the pathology simulation in the gums of the molars of the mandible of rats in a dose of 0.1 ml. The rats were removed from the experiment under thiopental anesthesia by bleeding from the heart in three stages: 2 weeks, 4 weeks and 6 weeks after injections of hyaluronic acid preparations with mannitol. Gums were removed from the injection site. The con-tent of malonic dialdehyde with thiobarbituric acid (MDA) [2], elastase activity by hydrolysis of Nt-BOC-L-alanin-p-nitrophenyl ester [3] and acid phosphatase (AP) by hydrolysis of para-nitrophenyl phosphate at pH 4.8 [2]. Static processing
ЕКСПЕРИМЕНТАЛЬНО-ТЕОРЕТИЧНИЙ РОЗДІЛ
© Ларкина С. А., Селецкая А. В., Макаренко О. А., 2019.
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“ В і с н и к с т о м а т о л о г і ї ” , № 1 , Т 3 1 - 2019
of obtained results was carried out according to the standard Student-Fisher method.
Results. Studies have shown that disposable injections of 1.4 %; 2 % and 3 % of hyaluronic acid with mannitol 0.9% in the gums of rats with parodontitis have antioxidant (Malondialdehyde level) and membrane-protective (acid phosphatase activity) ef-fect, more pronounced after 4-6 weeks. At the same time, the lowest values of the studied markers of inflammation were rec-orded in the gums of rats 6 weeks after the injection of 4 % hya-luronic acid with mannitol 0.9 %. 2 weeks after the injection of hyaluronic acid preparations with mannitol, regardless of its concentration, development of inflammation (increased elastase activity) is noted, the degree of which exceeds rats with parodontitis. The intensity of inflammation decreases after 4 weeks or more significantly 6 weeks after injection. The com-plete disappearance of inflammation in the gums, normalization of elastase activity was registered only 6 weeks after injection of the preparation containing 4 % hyaluronic acid with mannitol 0.9 % compared with progressive parodontitis in uninjected rats.
Conclusions. The data obtained indicate that hyaluronic acid with mannitol has antioxidant and membrane-protective action, as well as anti-inflammatory efficacy.