MORPHOLOGICAL STUDIES OF BONE STRUCTURES DURING ORTHODONTIC EXTRUSION OF TEETH IN EXPERIMENTAL CONDITIONS
DOI:
https://doi.org/10.35220/2078-8916-2019-33-3-2-7Keywords:
bone tissue, orthodontic extrusion, animalsAbstract
Purpose of research. To study the state of bone tissue in orthodontic extrusion of teeth by directed osteoregeneration arc 0,016 Niti.
Material and methods. Bone tissues of jaws of animals (rabbits-males of the Dutch breed at the age of 16-18 months) served as a material for morphological research. The choice of rabbits of this age is due to the fact that at this age the cells that cause the constant growth of teeth cease to function. In total we used 10 animals. For ortho-dontic extrusion, we used the appropriate scheme and the extrusion period was 6 weeks.
After this period, the animals were withdrawn from the experiment, periodontal tissue and bone tissue were fixed in a 10% solution of neutral formalin for 72 hours, after which the tissue samples were washed in running water for 24 hours.
Research results and their discussion. Histological ex-amination of micropreparations revealed that the hole of the removed tooth is filled with bone tissue. In the study of drugs of this study group revealed that the marginal and alveolar gums are covered with a multilayer flat keratin-ized epithelium and there is an unexpressed thickening of the stratum corneum against the background of a tenden-cy to thin the spiny and granular layers.
Histological examination of the materials revealed that the bone tissue is represented by the cortical plate and spongy bone. The interstitial space is filled with a reticu-lar stroma without foci of hematopoiesis and with areas of coarse-fibrous unformed connective tissue. Cortical bone with pronounced gaversovyh channels presented lamellar bone tissue.
Summary. We have established and morphologically con-firmed that the guided osteoregeneration using the arc Niti 0,016 does not lead to a complete filling of the bone tissue of the hole of the removed tooth.
It was found that an additional damaging factor in this case is the development of tissue hypoxia, as evidenced by the presence of ischemic zones in the surrounding soft tis-sues, up to the development of sclerotic changes in the mucosal lamina proper.
References
Efficacy and predictability of short dental implants (<8mm): a critical appraisal of the recent literature / M. Srinivasan, L. Varquez, P.Rieder [et al.] // Int J Oral Maxillofac Implant. – 2012. – №27. – Р. 1429-1437.
Steenberghe D. The clinical use of deproteinized bovine bone mineral on bone regeneration in conjunction with immediate implant installation / D. Steenberghe, A. Callens, L. Geers // Clin. Oral Implants Res. – 2000. – V.1, №2. – P. 171-178.
Pye A. D. A review of dental implants and infection/ A. D. Pye, D. E. Lockhart, M.P. Dawson // J Hosp Infect. –2009. – №2(72). – Р. 104-10.
Хабкек Д. Руководство по дентальной импанто-логии / Д. Хабкек, Р. Уотсон, А. Сизн // М: Медпресс-инфо.: – 2010. – 224 с.
Ушаков А. И. Планирование дентальной им-плантации при дефиците костной ткани и профилактика
операционных рисков / А. И. Ушаков, Н. С. Серова, А. В. Даян // Стоматология. – 2012. – № 1. – С. 48-53.
Preservation of alveolar bone in extraction sockets using bioabsorbable membranes / V. Lekovic [et al.] // Journal of Periodontology. – 1998. – №9(69). – Р. 1044-1049.
Socket Augmentation: Rationale and Technique / Hom-Lay Wang. [et al.] // Implant Dentistry. – 2004. – № 4 (13). – Р. 286-296.
Changes in alveolar bone height and width following ridge augmentation using bone graft and membranes / BJ. Si-mon [et al.] // Journal of Periodontology. – 2000. – №11 (71). – Р. 1774-1791.
Implant site development by orthodontic extrusion: a systematic review / M. Korayem, C. Flores-Mir, U. Nassar [et al.] // Angle Orthod. – 2008. – № 78. – Р. 752 − 760.
Саркисов Д. С. Мікроскопічна техніка: Керівництво. / Д. С. Саркисов, Ю. Л. Перов. – М: Медици-на. – 1996. – 544 с.
Атлас электронной микроскопии по частной гис-тологии / [Баринов Э. Ф. и др.]. – Донецк : Изд-во Донецко-го медицинского ун-та, 1998 . Т. 2, 1998. – 271 с. 12. Шереметьева Г. Ф. Методы гистологических исследований / Г. Ф. Шереметьева, Е. З. Кочарян. – РАМН, Научный центр хирургии. – М. – 1995. – 38 с.
