EXPERIMENTAL MODELING OF THE ABNORMAL TIMING OF DENTITION

Abstract

The aim of the study. The study is dedicated to the investigation of the influence of L-thyroxine, Mercazolil, and antibiotics (Amoxiclav and Cefoperazole) received antenatally on the timing of molar eruption in rats. Materials and methods. The experiment was performed on 20 white female laboratory rats and 37 male rats. Starting from the first day of drug administration, the males were put together with females. Depending on the drugs used, the animals were divided into 4 groups: 1. Intact (vivarium diet); 2. L-thyroxine in a dose of 10 mg/kg + vivarium diet; 3. Antibiotics (cefoperazone 180 mg/kg – pregnancy, amoxiclav 135 mg/kg – lactation) + vivarium diet; 4. Mercazolil – (20 mg/kg – pregnancy), (50 mg/kg – lactation) + vivarium diet. Further studies were conducted on little rat that were born from the females receiving these drugs. Rats were removed from the experiment under thiopental anesthesia (20 mg/kg) after the lactation period at approximately 35 days of age. The total duration of the experiment was 56 days. During the experiment, the date of the beginning of dentition and the degree of eruption were determined in little rat. Results of the study. The first molar erupted on 15.42±0.16 days in rats of the 2nd group. The eruption of the second molar in the rats of the 2nd group occurred 1.96 days earlier than in those of the 1st group. Eruption of the third molar occurred 2.89 days earlier than in the first group. Thus, the first molar in the 3rd group erupted 2.86 days later than in the rats of the 1st group. The second molar in this group of rats erupted 2.16 days later than in the rats of the intact group. The third molar in rats of the 3rd group erupted 2.13 days later than in rats of the intact group. The first molar in the rats of the 4th group erupted 3.43 days later than in those of the intact group. The second molar erupted 4.14 days later than in the intact group of rats. Eruption of the third molar occurred 3.15 days later than in the rats of the first group. Conclusions. It was established that early eruption of molars occurred in rats born from the females with simulated experimental hyperthyroidism during pregnancy and lactation. As a result of experimental studies, it was proved that dentition was delayed in rats born from the females that were administered antibiotics orally during pregnancy and lactation. It was established that in rats born from the females that were modelled experimental hypothyroidism during pregnancy and lactation, the eruption of molars was delayed.