ASSESSMENT OF THE EFFECT OF A TREATMENT-ANDPROPHYLACTIC COMPLEX DEVELOPED FOR CHILDATHLETES ON “LIVER” MARKERS AND INDICATORS OF LIPID METABOLISM IN BLOOD SERUM, THE STATE OF INTESTINAL MICROBIOCENOSIS AND ENDURANCE OF RATS

Abstract

The data of the scientific literature of the last decade proved that among athletes only 15–28 % of the examined are healthy. Intense physical and psycho-emotional stress leads to the development of overtraining syndrome due to a decrease in immunological reactivity and adaptive capacity of the organism. Contributes to the emergence of stress immunodeficiency and impaired nutrition of the athlete. Therefore, there is a need to develop comprehensive measures in the program for the prevention of general somatic pathology, including dental diseases, taking into account the peculiarities of its pathogenesis in children actively involved in sports. The aim of the work was to experimentally evaluate the effect of a therapeutic and prophylactic complex developed for child athletes on liver markers, lipid metabolism in blood serum, the state of intestinal microbiocenosis and endurance in rats under conditions of a combination of malnutrition and physical activity. Materials and methods. The experiment was performed on 25 white rats of herd breeding, divided into 3 levels of the group: 1 – intact; 2nd – inadequate nutrition; 3rd – inadequate nutrition + therapeutic and prophylactic complex per os (egg albumin plus, biotrit-C, bactoblis, apple pectin, vitamin-mineral complex “Alphavit”). Rats of all groups were additionally subjected to physical activity by repeated presentation of swimming load weighing 10 % of body weight. After the end of the experiment in the blood serum of rats, “liver” markers, indicators of lipid metabolism, and the content of malondialdehyde were determined; the activity of urease and lysozyme was determined in the homogenates of the mucous membrane of the small and large intestine, the degree of dysbiosis was calculated, and the endurance of rats was evaluated. Research results. The experimental study conducted allows us to conclude that inadequate nutrition against the background of physical activity leads to the destruction of hepatocytes and disruption of their function, contributes to a decrease in antimicrobial protection in the mucous membranes of the small and large intestines and the development of dysbiosis, leads to a decrease in the level of triglycerides in the blood serum, and also significantly, reduces the endurance of rats during exercise. It is shown that the use of the proposed treatment-andprophylactic complex makes it possible to prevent these negative changes. Conclusion. The results of experimental studies testify to the antioxidant, antidysbiotic, certain hepatoprotective efficacy of the treatment-and-prophylactic complex and its ability to increase the adaptive capabilities of the rat organism in conditions of experimental pathology.