ROLE OF POLYMORPHISM OF DETOXIFICATION, AMELOAND DENTINOGENESIS GENES IN THE DEVELOPMENT OF MULTIPLE CARIES IN HYPOXIA, IODINE AND FLUORIDE DEFICIENCY
DOI:
https://doi.org/10.35220/2078-8916-2022-46-4.2Keywords:
genetic polymorphism, multiple caries, hypoxia, iodine and fluoride deficiencyAbstract
Purpose of the study. Study of the effect of cyp1a1 detoxification gene polymorphism A1506G (Ile462Val), GSTM +(0), amelogenesis amelx T>C rs17878486, and viz xu49xue Pro17Ser dentinogenesis on the etiology and susceptibility to multiple caries in children with hypoxia, iodine and fluoride deficiency. Materials and methods. Genotyping was performed in 15 children with multiple caries aged 2 to 6 years, who live in the mountainous region of the Transcarpathian region in conditions of iodine and fluoride deficiency. The comparison group consisted of 10 children of the same age with low intensity of carious lesions living in the same area. The biological material for the study was LPM extracted from buccal epithelial cells. A study of the deletion polymorphism of the glutathione S gene transferase M1 revealed a significant increase in the proportion of allele dysfunction in the group of children with multiple caries compared with the group of children with low caries (46.7 % and 20 %, respectively). Functionally defective C allele of the Amelx T> C rs17878486 gene was 23.3 % in the main group and 15 % in the comparison group. Functionally defective allele of the T gene c. 49C> T of the DSPP gene is present in 16.7% of children in the main group and in 10 % of children in the comparison group. As a result of the molecular genetic study of the polymorphic locus T> C of the amelogenin gene (AMELX) and the locus c. 49C> T of the DSPP gene revealed a tendency to the protective effect of caries of functionally complete genotypes of these genes relative to heterozygous genotypes (OR = 1.52 and 1.80, respectively). The genes of detoxification, amelo- and dentinogenesis studied in this work in conditions of hypoxia, iodine and fluoride deficiency are not the only participants in such a complex process, but their study helps to identify risk groups in the preclinical stage to prevent tooth decay in early childhood.
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