EXPERIMENTAL EVALUATION OF PERIPHERAL BLOOD LEUKOCYTE DIFFERENTIAL IN RATS UNDER CHRONIC STRESS MODELING AND THERAPEUTIC AND PREVENTIVE MEASURES

Abstract

Chronic stress is a significant factor that negatively affects the immune system, contributing to the development of inflammatory processes and immunosuppression, which can worsen the condition of oral tissues and overall dental health. This emphasizes the need to develop effective therapeutic and preventive measures to correct stressrelated disorders. The purpose of the study was to evaluate the effect of a therapeutic complex of drugs on the leukocyte formula of peripheral blood of rats under conditions of chronic stress modeling. Materials and methods. The study involved 34 male Wistar rats aged 2 months. The animals were divided into three groups: an intact (control) group, a group with a model of chronic sound stress, and a group with a model of chronic sound stress where the treatment complex was applied. Chronic stress was modeled using an ultrasonic repeller that generated sounds of different frequencies in the ultrasonic and audible ranges. The treatment complex was administered orally during the experiment. At the end of the study, peripheral blood was collected for the analysis of hematological parameters and leukocyte formula according to standard methods. Research results. In rats exposed to chronic sound stress, significant changes in the leukocyte formula of peripheral blood were found: a decrease in the total number of leukocytes, an increase in the content of neutrophils, and a decrease in the percentage of lymphocytes and eosinophils. This indicates the development of inflammatory reactions and stress-induced immunosuppression. The use of the treatment complex led to the normalization of these indicators, restoring the number and ratio of leukocytes to the level of the intact group, indicating the restoration of the immune system. Conclusions. The therapeutic and prophylactic complex of drugs effectively reduces the negative impact of chronic stress on the immune system, normalizing the leukocyte formula of peripheral blood. This confirms the expediency of using this complex for the correction of immune disorders caused by stress factors, especially in dental practice, where maintaining immune competence is important.