EXPERIMENTAL EVALUATION OF ANTIOXIDANT ENZYMES IN RAT BLOOD SERUM UNDER THE INFLUENCE OF GINGIVITIS MODELLING AND TREATMENT AND PREVENTIVE MEASURES

Abstract

Chronic inflammation of the gums and the accompanying oxidative stress remain among the pressing issues in contemporary biomedical science. Gingivitis is associated with the activation of lipid peroxidation (LPO) and an increase in protease activity, particularly elastase, which exacerbates damage to periodontal tissues and destabilizes local immune defense. Considering the crucial role of antioxidant mechanisms in preventing chronic inflammation, the introduction of therapeutic and prophylactic measures aimed at reducing oxidative stress and modulating the inflammatory response is viewed as promising. The purpose of the study was to evaluate the effect of a therapeutic complex of drugs on blood serum inflammation markers under gingivitis modeling and prophylactic measures. Materials and methods. The study involved 30 male Wistar rats of 1 month of age, with an average weight of 65-75 g at the beginning of the experiment and 200-210 g at the end of the experiment. The rats were divided into 3 groups. The material for biochemical studies was the blood serum of white rats. At the end of the study, the serum concentration of malondialdehyde (MDH) and elastase activity were measured. A statistically significant difference between alternative quantitative features with a distribution corresponding to the normal law was evaluated using Student’s t-test. The difference was considered statistically significant at p<0.01. Research results. n the gingivitis group, MDH levels increased by 38.2 % and elastase activity by 42.8 % compared with intact animals, indicating the development of systemic inflammation and LPO activation. Administration of the therapeutic-prophylactic complex over a 60-day period led to a 21.1 % reduction in MDH levels and a 26.2 % decrease in elastase activity compared to the “Gingivitis Model” group, bringing these parameters close to those of the intact group. Conclusions. The proposed drug complex demonstrates a pronounced anti-inflammatory and antioxidant effect under experimental gingivitis in rats, as evidenced by decreased MDA levels and elastase activity. This approach holds promise for the prevention and treatment of chronic inflammatory periodontal diseases, given its proven ability to inhibit pathological lipid peroxidation processes and inflammatory reactions.