THE ROLE OF ORAL FLUID BOIMARKERS IN THE DIAGNOSIS OF INFLAMMATION IN THE DYNAMICS OF SURGICAL TREATMENT OF MANDIBULAR FRACTURE IN CHILDREN

Abstract

The purpose of this study. Study of the role of matrix metalloproteinase-8 and klotho protein expression in the diagnosis and prognosis of inflammation in children with mandibular angle fracture in the dynamics of surgical treatment. Material and research methods. We conducted a study of children with a mandibular angle fracture aged 6 to 17 years, who were divided into 2 groups depending on age. The comparison group consisted of healthy children who did not have dental pathology and were clinically healthy. All the examined children were students or students of educational institutions and applied for advice and treatment in the Department of Maxillofacial Surgery. Biochemical studies were performed in the patients’ oral fluid. Statistical data processing was performed using Generally Accepted nonparametric methods using MS Excel and the licensed statistical program Biostat. Differences between groups with an error probability of less than 5 % (p<0.05) were considered reliable. Research results and their discussion. Destruction of the alveolar bone occurs due to the degradation of components of the extracellular matrix and leads to irreversible bone loss. According to a number of authors, matrix metalloproteinases (MMPs) play an important role in this pathological process. Thus, in the oral fluid of children aged 6-11 years, the concentration of MMP-8 one week after surgical treatment of a mandibular angle fracture was 3.18±0.17 ng/mL, and in the older age group of 12-17 years – 3.93±0.23 ng/mL and these indicators significantly differed and depended on the age of the child (p<0.05). One month after surgical treatment of the lower jaw fracture in children in both age groups, the digital values of both klotho protein and MMP-8 significantly changed. Conclusions. Our studies have shown the functional importance of such proteins as matrix metalloproteinase-8 and klotho protein in the pathogenesis of inflammatory processes in the jaw and this is due, in our opinion, to local stimulation of regenerative processes, since autoplasm works as a natural stimulator of regeneration processes due to the release of a large number of growth factors formed during blood clotting during platelet destruction and improvement of microcirculation. It can be assumed that this class of peptides, which are usually secreted in response to a jaw fracture and are biomarkers for determining the presence of an inflammatory process as such, its stage, and monitoring the results of treatment.