ESTIMATION OF PRO-INFLAMMATORY CYTOKINES LEVELS IN GINGIVAL CREVICULAR FLUID AND MARKERS OF BONE METABOLISM IN BLOOD SERUM IN PATIENTS WITH GENERALIZED PERIODONTITIS AFTER PROSTHETIC TREATMENT
DOI:
https://doi.org/10.35220/2078-8916-2023-50-4.14Keywords:
generalized periodontitis, prosthetic treatment, efficacy, pro-inflammatory cytokines, markers of bone metabolism, biochemical research, ELISA.Abstract
Purpose of the study. To estimate pro-inflammatory cytokines levels in gingival crevicular fluid and markers of bone metabolism in blood serum in patients with generalized periodontitis after prosthetic treatment. Research methods. During this work, metal-ceramic splints-dentures were made for 30 patients with generalized periodontitis of the I-II degree of severity, chronic course, aged 32-45 years, equally men and women. The clinical and laboratory examination of the patients was carried out before the beginning of prosthetic treatment, after preparation of supporting teeth and in the near term after prosthetic treatment (in 2-3 days after fixation of designs) and in its long term (in 6 and 12 months). To estimate changes in periodontal tissues, the dynamics of proinflammatory cytokines MMP-8, IL-1β, IL-6, and TNF-α in gingival crevicular fluid samples were studied. As markers of bone metabolism, serum levels of tartrate-resistant acid phosphatase (TRAP), bone alkaline phosphatase (BAP), osteocalcin, and C-terminal pro-peptide of type I procollagen were determined. Scientific novelty. According to biochemical research, prosthetic treatment gives destabilization of the periodontal status in the near term of observation, in particular after preparation of teeth for metal-ceramic splint-dentures. Injury to the marginal periodontium causes a rapid increase in the level of proinflammatory interleukins IL-1β, IL-6, and TNF-α from the initial values (P < 0.001), while MMP-8 level in the gingival fluid increases more slowly, reaching the highest values in 2-3 weeks. After cementation of fixed dentures, the levels of pro-inflammatory cytokines normalize, and in 6 and 12 months they are same as the initial values (P > 0.05). In the long term, there is an improvement in clinical signs compared to the data at the beginning of treatment. It was established preservation of bone mineral density (BMD) levels according to computer tomography data (P > 0.05). There were no significant differences in indicators of bone metabolism markers at different periods of observation (P > 0.05). Conclusions. According to the results of the conducted clinical and laboratory research, prosthetic treatment of patients with generalized periodontitis allows to stabilize the periodontal tissues state and to stop the inflammatory-destructive process.
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